Frozen storage of mouse embryos by vitrification.
نویسندگان
چکیده
منابع مشابه
Survival and transfer test of mouse early embryos frozen by vitrification method
An approach to organ cryopreservation, namely, vitrification has been review ed by FAHY et al.1). RALL and FAHY2) obtained some promissing results using 8-cell mouse embryos indicating the feasibility of obtaining high survival following rapid freezing by vitrification. However, there have been only a preliminary report2) on the feasibility of obtaining offspring by the vitrification method. Th...
متن کاملWhich stage of mouse embryos is more appropriate for vitrification?
Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...
متن کاملVitrification of Mouse and Sheep Embryos
Vitrification is a method of storing cells in a supercooled state, and is particularly useful for the cryostorage of embryos. With a supply of liquid nitrogen and simple apparatus, vitrification in the field situation is feasible and is potentially simpler and cheaper than the standard freezing processes. However, survival of embryos after vitrification has not yet proved better than after free...
متن کاملCryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
Cryopreservation of mouse embryos is a technological basis that supports biomedical sciences, because many strains of mice have been produced by genetic modifications and the number is consistently increasing year by year. Its technical development started with slow freezing methods in the 1970s(1), then followed by vitrification methods developed in the late 1980s(2). Generally, the latter tec...
متن کاملEfficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device
BACKGROUND Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART. METHODS In Experiment...
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ژورنال
عنوان ژورنال: The Japanese journal of animal reproduction
سال: 1987
ISSN: 0385-9932
DOI: 10.1262/jrd1977.33.77